Electron paramagnetic resonance studies on the cytochrome oxidase from yeast

Electron paramagnetic resonance studies on the cytochrome oxidase from yeast.

I M. D. KAMEN, R. G. BARTSCH, T. HORIO AND H. DE KLERK, in S. P. COLO\VICK AND N. O. tXAPLAN, Methods in Enzymology, Vol. VI, Academic Press, New York, I963, p. 391. 2 T. HORIO AND M. D. KAMEN, Biochim. Biophys. Acta, 48 (I96I) 266. 3 G. COHEN-BAZIRE, ~V. R. SISTROM AND R. Y. STANIER, J . Cellular Physiol., 49 (1957) 25. 4 R. G. BARTSCH, in H. (;EST AND A. SAN PIETRO, Bacterial Photosynthesis, ...

Electron paramagnetic resonance studies on the ferredoxin from Clostridium pasteurianum.

We have recently demonstrated an EPR signal in spinach ferredoxin on reduction either enzymically or with dithionite (Palmer and Sands, 1966). It is the purpose of this communication to report on the EPR spectrum found in the ferredoxin obtained from the bacterium C. pasteurianum. The ferredoxin was purified by the method of Mortenson (1964) and had an absorbancy ratio 285 rnp; 3QO rnF of 0.82....

The nature of the phosphate complex of sulphite oxidase from electron-paramagnetic-resonance studies.

The phosphate complex of sulphite oxidase in the Mo(V) oxidation state was investigated by e.p.r. spectroscopy. Third-derivative spectra reveal a wealth of structural detail previously unobserved in this spectrum. Most notable is the presence of hyperfine coupling from two inequivalent I = 1/2 nuclei, which we tentatively attribute to two 31P nuclei. Unresolved hyperfine interactions from at le...

Electron Paramagnetic Resonance Study of the Interaction of Some Anionic Ligands with Oxidized Pseudomonas Cytochrome Oxidase*

Sodium fluoride induces changes in the heme dl components of both the frozen solution EPR and room temperature optical absorption spectra of oxidized Pseudomolurs cytochrome oxidase. The EPR resonances of the heme dl (iron-chlorin) moiety(ies) appear to undergo a rhombic to axial transition ( g = (2.52, 2.42, 1.73) 4 (2.54,2.54, 1.61)) with increasing concentrations of fluoride (0 3 0.1 M, pH ...

Electron paramagnetic resonance study of the interaction of some anionic ligands with oxidized Pseudomonas cytochrome oxidase.